Figure 3
From: Ex vivo promoter analysis of antiviral heat shock cognate 70B gene in Anopheles gambiae
Induction of the hsc70B promoter in transfected Vero cells by ONNV infection. The constructs containing the 2.6 kb and 2.2 kb-long 5' upstream regions were evaluated for hsc70B promoter activity during ONNV infection (MOI = 0.001). The luciferase activity was measured at 60 h post ONNV infection when CPE became apparent. The hsc70B promoter activity, as measured by relative luciferase activity (mean ± SD), was significantly elevated in both constructs when compared to uninfected controls (2.2 kb uninfected vs. infected: P < 0.01, t = 4.702, df = 6; 2.6 kb uninfected vs. infected: P < 0.01, t = 5.681, df = 6).