Non coding extremities of the seven influenza virus type C vRNA segments: effect on transcription and replication by the type C and type A polymerase complexes

Table 4 Analysis of the CAT vRNA and mRNA levels by real-time RT-PCR

Polymerase complex^a	vRNA template^b	Ct vRNA^c	Ct mRNA^c
none	PB2	34.2 ± 0.7	38.4 ± 1.5
	PB2/6U	34.6 ± 1.1	39.0 ± 1.4
	PB2/24	33.6 ± 0.6	39.2 ± 0.6
	PB2/24 mu	32.9 ± 0.7	37.8 ± 1.1
	M	33.5 ± 1.0	37.9 ± 0.8
Type C	PB2	20.5 ± 0.6	23.0 ± 1.1
	PB2/6U	18.6 ± 1.4	18.3 ± 0.6
	PB2/24	20.0 ± 0.5	19.8 ± 1.3
	PB2/24 mu	19.1 ± 0.6	20.4 ± 0.8
	M	19.3 ± 1.2	19.5 ± 1.5
Type A	M	19.6 ± 1.1	19.5 ± 1.4

^a: 293T cells were transfected in duplicate either with plasmid pHMG alone (no polymerase complex) or with the four pHMG plasmids encoding the polymerase complex of type C or type A influenza virus.
^b: vRNA templates were derived from the M or PB2 (with wt or mutated 5' non coding sequences) segments of the influenza virus type C.
^c: Values are means ± SD of six independent cDNA syntheses from two independent transfections (three cDNAs per transfection).

ISSN: 1743-422X