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Figure 4 | Virology Journal

Figure 4

From: Neutralizing human monoclonal antibody against H5N1 influenza HA selected from a Fab-phage display library

Figure 4

H5N1-VLP neutralization characteristics of αHA Fab IgG. (A) To confirm the neutralization observed with the indicated α-HA Fab IgG, neutralization assays were repeated. IN597 and VN04 were produced with the HA2 of VN04 while QH and INDO05 were produced with their respective HA2 in HEK293 cells and the media incubated with 2 ug/ml of the indicated IgG for 60 min prior to addition to MDCK cells. Fusion of the VLP with MDCK was determined by measuring the number of cells producing GFP by flow cytometry with the control assay with no IgG taken as 100%. (B) Dose dependant neutralization was assessed against IN597 H5N1-VLPs, with 8 ug/ml and serial dilutions of the indicated antibodies, as described above. The humanized protective antibody VN04-2 was used as a positive control.

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Virology Journal

ISSN: 1743-422X