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Figure 2 | Virology Journal

Figure 2

From: Adaptation to cell culture induces functional differences in measles virus proteins

Figure 2

Effect of mutations in the P, C and V proteins of MeV on mini-genome replication. (A) CV-1 cells were infected with MVAT7 and transfected with pMV107(-)CAT, pTM1-D87-wt N, pTM1-D87-wt L and the indicated plasmids expressing P proteins. (B, C) CV-1 cells were infected with MVAT7 and transfected with pMV107(-)CAT, pTM1-D87-wt N, pTM1-D87-wt P, pTM1-D87-wt L and 1 μg of the indicated plasmids. For the negative controls, pTM1-D87-wt L was omitted. The amount of transfected DNA was kept constant through the addition of pTM1 vector. CAT protein production in cytoplasmic extracts of quadruplicate samples was measured by ELISA. The amount of CAT protein measured in the presence of pTM1-D87-wt P and absence of C- or V-expressing plasmids was set to 100% in each panel. Each panel shows the average of three independent experiments. Error bars denote one standard deviation. (*: P ≤ 0.01, **: P ≤ 0.001)

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