Bootscan analysis of the full-length sequence of the isolate PO1 against Luteoviridae members. The horizontal axis represents the nucleotide distance of the midpoint of the window (covering 100 pb). The vertical axis represents the percentage of trees (using 100 bootstrap replicates) that support branching with the divergent isolate query sequence. The values for one Enamovirus (PEMV-1), one Luteovirus (BYDV-MAV), the four Polerovirus with the highest bootstraps (TYV, CYDV-RPV, BWYV, and BMYV), and TVDV (an unclassified member of the family) are represented. The GenBank accession numbers for these sequences are listed in the Material and Methods section.