Level of proinflammatory cytokines (TNFα, IFNγ, MCP-1, IL-6, IL-10 and IL-12p70) in plasma of mice at different hours post infection (HPI). At various time point three mice from both infected as well as control mice were bled intra orbitaly in EDTA. The plasma was separated by centrifugation and 50 μl was used for quantitation of cytokines. The cytokines were quantitated by using mouse inflammatory beads assay. The sensitivity of this assay is 0–5000 pg/ml. The values are Mean ± SE of three experiments. Each experiment plasma from three mice was processed separately at different post infective hours. *p < 0.05, **p < 0.01, *** p < 0.000.