Molecular and macromolecular alterations of recombinant adenoviral vectors do not resolve changes in hepatic drug metabolism during infection

Table 1 Oligonucleotide primer sequences and amplification conditions for RT-PCR analysis of hepatic CYP and related nuclear receptors^a

Gene	Sequence (5'-3')	PCR product (bp)	PCR conditions
			Cycle	Annealing temperature (°C)
CYP3A2	Sense: TTG ATC CGT TGT TCT TGT CA Antisense: GGC CAG GAA ATA CAA GAC AA	323	23	60
CYP2C11	Sense: CTG CTG CTG CTG AAA CAC GTC Antisense: GGA TGA CAG CGA TAC TAT CAC	249	22	60
PXR	Sense: GAG CTC TGG GCA GAA ACA TC Antisense: ACA CGG CAG ATT TGA AGA CC	217	29	62
RXRα	Sense: CTC TAC CCA GGT GAA CTC TT Antisense: TGC TGC TCA CAG GGT TCA TG	293	30	58.4

^aPrimer sequences for RXRα were obtained from Macejova et al. [57]. All other sequences were designed in our laboratory as described previously [9].

ISSN: 1743-422X