Figure 6

Hepatic CYP2C11 mRNA levels are Significantly Reduced for at Least Four Days After A Single Dose of Active Adenovirus. Mean relative intensities of RT-PCR products and representative agarose gels 0.25 (A), 1 (B), 4 (C), and 14 (D) days after treatment with wild type virus (WT), first generation recombinant adenovirus expressing beta-galactosidase (AdlacZ), PEGylated AdlacZ, (PEGAd), helper-dependent virus (HDAd), or inactivated AdlacZ (UVAd). mRNA levels are reported as band densities of gene-specific RT-PCR products with respect to the density of products from an internal control (18S rRNA) in arbitrary units. For all panels, animals dosed with phosphate buffered saline served as controls (Vehicle). Values are presented as the mean ± standard error of 4 animals/treatment/timepoint. Statistical significance was determined between individual treatment groups and vehicle controls by one-way analysis of variance with a Bonferroni/Dunn post-hoc analysis. *p ≤ 0.05, **p ≤ 0.01.