Figure 9
From: Discovery of frameshifting in Alphavirus 6K resolves a 20-year enigma
Detection of SFV 6K and/or TF proteins for WT and TF- viruses. (A) SFV-infected cells were labelled with [35S]Met/Cys and cell lysates (1 hr and overnight, o/n) and purified virions were analyzed by SDS-PAGE. Equal amounts of transfecting RNA and cells were used for each sample. Lanes 1–4 and 7–8 are from the same gel, lanes 5–6 are from a separate gel; Phospho-Imager, 2 days exposure. Negative controls are shown in Figure 8. (B) As above, but with higher sample loading.