Figure 10
From: Discovery of frameshifting in Alphavirus 6K resolves a 20-year enigma
Immunoprecipitation of SFV 6K and/or TF proteins for WT and TF- viruses. SFV-infected cells were labelled with [35S]Met/Cys and cell lysates (1 hr and overnight, o/n) and purified virions were subjected to immunoprecipitation with Ab-6KTF-N or Ab-TF-C followed by SDS-PAGE, and exposed on x-ray film for 3 weeks. Equal amounts of transfecting RNA and cells were used for each sample.