Figure 1
From: Iota-Carrageenan is a potent inhibitor of rhinovirus infection
Carrageenans promote cell viability of HRV2 infected HeLa cells and inhibit HRV2 replication in vitro. A. HeLa cells grown in 96-well plates were infected with HRV2 (0,1 TCID50/cell) in the presence of Carrageenans (different types are indicated at the x-axis) at a concentration of 200 μg/ml. Plates were incubated at 37°C until cells in the control (no polymer added) showed >90% damage. Cell proliferation was determined with an XTT-assay. OD values (492 nm) obtained from mock infected cells (compare x-axis) were set to 100%, and the viability of cells infected in the absence of polymer was set to 0% (y-axis). The bars represent the mean of a quadruplicate experiment, the standard deviation is indicated. B. HeLa cells in 24-well plates were infected with HRV2 (0,1 TCID50/cell) in the presence of Carrageenans (different types are indicated at the x-axis) at a concentration of 200 μg/ml. Viral infectivity in the supernatants was determined by TCID50 assay on HeLa cells (y-axis). Values represent the mean of six parallel titrations, standard deviation is indicated.