Figure 3

Phylogenetic trees of all viral E1/E2 amino acid sequences encompassing the HVR1 within each fraction. The phylogenetic tree was constructed with the Treecon software and rooted with the unique IgG-enriched species [EU482135]. The genetic distance is shown as a scale bar. A bootstrap analysis using 100 bootstrap replicates was performed to assess the reliability of each branch point. Bootstrap scores are given as percentage value. The values greater than 70% are annotated at appropriate branches. Different font colours are used to represent the different fractions: red for the unfractionated serum species, blue for the IgG-depleted species and green for the IgG-enriched species, [EU482135] was also the subdominant species of the unfractionated fraction. The species with the insertion event are represented in the figure by an asterisk (*) and the species with the indel event are represented by 2 asterisks (**).