Figure 2

Specificity and levels of gene expression mediated by BacFLT-GFP. (a) Drawing showing the cassette structure of BacFLT-GFP. Abbreviations: hFlt-1, promoter sequence of the human flt-1 gene (nucleotides -748 to +284 bp); EGFP, enhanced green-fluorescent protein; BGHpA, bovine growth hormone poly adenylation sequence. (b) (Left panel), Representative histogram obtained by flow cytometry 48 h after transduction of BUVEC-E6E7-1 cells with 100 MOI of BacFLT-GFP and 5 mM of butyrate. The percentage of GFP+ cells is reported in the inset, and was calculated by subtracting the background from mock transduced cells (see Methods). (Right panel) Levels of expression obtained with BacCMV-GFP and BacFLT-GFP in BUVEC-E6E7-1 cells, representative of four independent experiments, mean ± SD. (c) Mean fluorescence intensity (MFI) measured 48 h after transduction with BacFLT-GFP relative to BacCMV-GFP. The percentage of GFP+ cells are indicate above of each bar. Cells were transduced with 100 MOI of BacFLT-GFP or BacCMV-GFP. Data are from four independent experiments ± SD.