Figure 1

Proviral integration, CD3 surface expression and relative CD3 gene expression over time after HTLV-I infection of WE17/10 cells. A, HTLV-I proviral genome analyses of WE/HTLV cell line by Southern blot. the complete provirus probe was hybridized to the WE/HTLV (at 3 weeks, 4 and 7 months p.i.) genomic DNA digested with Eco RI. B, the Kpn I fragment probe was hybridized to the (at 7 months p.i.) genomic DNA digested with Sac I. C, the Kpn I fragment probe was hybridized to the (at 7 months p.i.) genomic DNA digested with Eco RI. MT-2 and uninfected WE17/10 cell lines were used as positive and negative control respectively. D, TCR/CD3 surface expression over time after HTLV-I infection of WE17/10 cells. profiles showing the distribution of immunofluorescence from anti-CD3 antibody staining in a parallel antibody labeling experiment. Uninfected and HTLV-I infected cells were thawed from the frozen cell line bank at 5, 10, 40, 48, and 58 weeks p.i. TCR/CD3^low cells are identified as cells that fall below the minimum fluorescence intensity defined by the positive control but do not lie within the region defined by the negative control. TCR/CD3^hi cells fall within the region defined by mock-infected cells, and TCR/CD3^- cells fall within the region designated by the negative control. E, Histograms representation of relative CD3 gene expression in HTLV-I infected cells at various times p.i. determined by real time RT-PCR in relation to the percentage of surface TCR/CD3⁺ cells determined by flow cytometry. All percentages were calculated relative to uninfected cells (100% positive). GM-607 B cell line was used as a negative control.