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Figure 5 | Virology Journal

Figure 5

From: An ectromelia virus profilin homolog interacts with cellular tropomyosin and viral A-type inclusion protein

Figure 5

Coimmunoprecipitation of proteins that interact with ECTV-PH. His-tagged ECTV-PH was immunoprecipitated with mouse monoclonal anti-His antibodies and Protein G-Plus agarose along with any bound proteins from a BS-C-1 cell lysate. Proteins were separated by SDS-PAGE and stained with Coomassie blue. Lane 1, control immunoprecipitation; lysate contained no His-tagged ECTV-PH. Lane 2, proteins isolated from immunoprecipitation on cells expressing His-tagged ECTV-PH. Three bands at 16 kDa, 38 kDa and 84 kDa were excised from the gel and identified by mass spectrometry as indicated; a fourth band at 28 kDa was identified as a dimer of ECTV-PH in a western blot.

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Virology Journal

ISSN: 1743-422X