Figure 1
From: Porcine adenovirus type 3 E1Blarge protein downregulates the induction of IL-8
PAdV-3 E1Blarge inhibit IL-8 production. (A) Total RNA isolated at 6 h post infection of HeLa cells with indicated viruses was analyzed by RNA protection assay using RiboQuant Multi-Probe template set hCK-5. The protected band indicated by the label on the right migrate faster that undigested probes, as expected.(B). HeLa cells transfected with the human IL-8 promoter containing a NF-κB recognition sequence, cloned upstream from a luciferase reporter cDNA in the presence of plasmid pCDNA3.1 or pCDNA3.1-pE1BL were assayed for luciferase activity (expressed as relative light units [RLU]). The error bars represent the standard error of mean of triplicate samples.