Figure 1

COX inhibitors are negatively affecting MHV infection. (A) Caco-MHVR cells were incubated with culture medium (containing a concentration of DMSO similar to that present in the inhibitor solutions), 20 μM indomethacin, or 30 μM curcumine 1 h prior to inoculation with MHV-A59 (m.o.i = 0.01). The cells were maintained in the presence of the inhibitors until they were fixed at 6 h p.i. Infected cells were detected by an indirect IFA using an anti-MHV serum and Texas Red conjugated secondary antibodies. Fluorescence was viewed with a Nikon Eclipse E800 microscope. The numbers of MHV-infected cells in the drug-treated cells are presented as a percentage of the average number of infected cells in the mock-treated (control) cell cultures. Data are presented as mean ± standard error of mean (n = 6). For statistical analysis a one-way ANOVA with the Tukey-Kramer test was performed using GraphPad Prism version 3.00 for Windows (GraphPad Software). In all tests, P < 0.05 was considered statistically significant.