Complementation of NL4-EcoLys3 with E. coli tRNALys,3 mutants. Panel A. Diagram of E. coli tRNALys,3 T54G and T54C. The mutated nucleotide is indicated by a circle.
Panel B. Nucleotide diagram E. coli tRNALys,3 TG54GG. The mutated nucleotides are indicated by circles.
Panel C. Infectivity of NL4-EcoLys3 complemented by E. coli tRNALys,3, E. coli tRNALys,3 T54G, and E. coli tRNALys,3 TG5453CC mutants. 293T cells were co-transfected with 0.5 μg of proviral plasmid and with tRNA plasmids that were titrated in at the indicated quantities. Infectivity, for complementation of plasmid NL4-EcoLys3 with E. coli tRNALys,3 T54G is specified by open circle; NL4-EcoLys3 with wild type E. coli tRNALys,3 is specified by closed circle; NL4-EcoLys3 with E. coli tRNALys,3 TG5453CC is specified by open square. The infectivity is determined by the amount of luciferase determined by JC53-BL assay divided by amount of virus (p24 ELISA) to obtain RLu per nanogram. Values are the average (+SD) from three assays.