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Figure 5 | Virology Journal

Figure 5

From: Antisense RNA directed to the human papillomavirus type 16 E7 mRNA from herpes simplex virus type 1 derived vectors is expressed in CaSki cells and downregulates E7 mRNA

Figure 5

Western blot of E7 protein. Photograph of infected CaSki cell proteins electrophoretically separated in denaturing gels and reacted with a rabbit polyclonal antibody to HPV-16 E7 protein. HPV-16 positive CaSki cells were infected with R3659, R5225 or R5226 (1.0 p.f.u. per cell), harvested at 16 h.p.i., lysed, run on a 15% SDS-PAGE gel, transferred on a PVDF membrane, and probed first with the anti-HPV-16 E7 antibody and then with an HRP-conjugated anti-rabbit-Ig secondary antibody. The columns in the graph are: 1, CaSki cells with no HSV; 2 to 5, CaSki cells infected with wt HSV-1 (F), R3659, R5225, and R5226, respectively; 6, HaCaT cells (immortalized human keratinocytes with no HPV and thus no E7). The arrow indicates the location of the E7 protein in the gel.

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Virology Journal

ISSN: 1743-422X