Figure 3
From: Posttranslational N-glycosylation of the hepatitis B virus large envelope protein
N-glycosylation motifs within preS1 and preS2 of L are modified by N-glycosylation. HEK 293T cells were either mock-transfected (control) or with the wild-type and mutant L constructs, as denoted above each panel. Diagrams (Top) show the N-glycosylation sites (¥) remaining for each mutant and brackets ((¥)) indicate known partial usage at N309. Following transfection, cell lysates were mock-treated or digested with PNGase F prior to SDS-PAGE and L-specific immunoblotting analysis.