Figure 4
From: Structure-based engineering of papillomavirus major capsid L1: controlling particle assembly
Examination of particle assembly of L1 mutants by size-exclusion chromatography. The proteins were incubated in assembly buffer at 25°C for 30 minutes before analysis on the Superdex-200 column (16/60, Pharmacia) at a flow rate at 1 mL/min. The positions of the molecular standards are indicated by arrows. An HPV16 L1 construct, ΔN10+ΔC30, shown previously to assemble into T = 1 particles, was included as a control (blue line).