Figure 1

fAPN is efficiently expressed on BHK-21 cell surface via transient or constitutive expression. A) BHK-21 cells were transfected with fAPN/pcDNA3.1D/TOPO for 24 h and fAPN expression was stained with R-G-4 antibody post transfection. Transfection frequency was determined by counting 300 cells. B & C) BHK cell lines constitutively expressing fAPN and pCiNeo (vector only) were labeled with anti-fAPN monoclonal antibody R-G-4. fAPN expression frequency was determined by counting >300 cells.