Figure 1

Western analysis of HPV 31 L1. (Panel A). Cos-7 cells were transfected with pcDNA 3.1(-) expression vector expressing either wild type HPV 31 L1 or codon optimized HPV 31 L1. After 72 hours, lysates were harvested and analyzed by Western analysis with and antibody to L1. Lane A mock transfected Cos-7 cells. Lane B wild type HPV 31 L1. Lane C Codon optimized HPV 31 L1. Lanes D and E contain L1 peptides tagged with GFP and probed using a GFP antibody. Lane D wild type HPV 31 L1 N-terminus. Lane E wild type HPV 31 L1 C-terminus. (Panel B). Western analysis of in frame fusions to GFP of 198 base pair fragments from the C-terminal domain of wild type L1 following transfection into Cos-7 cells. Each fragment overlapped with the adjacent fragment by 99 base pairs. Following transfection cell extracts were analyzed by Western analysis using a GFP antibody. Lane A plasmid A, GFP fusion to nucleotides 6209–6407 Lane B fragment 2, nucleotides 6308–6506. Lane C fragment 3, nucleotides 6407–6605. Lane D fragment 4, nucleotides 6506–6704. Lane E fragment 5, nucleotides 6605–6803. Lane F fragment 6, nucleotides 6704–6902. Lane G fragment 7, nucleotides 6803–7063. Cartoon shows the fragments of L1 that were fused to GFP and examined in the experiments in panel C. Nucleotide numbers correspond to those in HPV 31 genome.