Comparison of recombinant JEV E protein expressed in deferent cells by Western blot. Cell lysate were resolved by 12% SDS-PAGE under non-reducing conditions. After electrophoresis proteins were transferred onto nitrocellulose membrane and probed with the antibody 2F2. ECL reagents were used to visualise protein bands. Lanes 1 to 6 are the cell lysate from Vero/ZF-ZR, Vero/JEF-JER, S2/ZF-ZR, S2/JEF-JER, Vero and S2 cells respectively.