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Figure 6 | Virology Journal

Figure 6

From: Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue

Figure 6

IFNβ induced in Ectocervical tissue by TLR ligands mediates resistance to HCMV. Ectocervical tissue was treated for 24 hours with TLR ligands. Tissues were washed three times and cultured for an additional 24 period in one ml of fresh medium. These conditioned supernatants were collected and incubated in the presence of either medium as a control, rabbit polyclonal anti-IFNβ antibody, or normal rabbit serum for 1 hour at 37°C. The treated supernatants were then transferred to wells of confluent HFF and cultured for 24 hours. The conditioned medium was removed and treated HFF were challenged with CMVPT30-gfp. Foci of infection containing gfp-expressing cells were then counted on day 10 post-infection. The data shown is the mean ± SD from one experiment that is representative of 3 independent experiments. ** indicates P ≤ 0.01 compared to control. *** indicates P ≤ 0.001 compared to control.

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Virology Journal

ISSN: 1743-422X