Figure 4

IFNβ induced by Poly I:C and LPS mediates resistance to HCMV in HFF. Monolayers of HFF were treated with either medium alone, Poly I:C (10 μg/ml) or LPS (10 μg/ml) for 24 hours. Cells were washed three times and cultured for an additional 24 period in one ml of fresh medium. These conditioned supernatants were collected and incubated in the presence of either medium as a control, rabbit polyclonal anti-IFNβ antibody, or normal rabbit serum for 1 hour at 37°C. Recombinant IFNβ (IFN) was also incubated in the presence of either medium as a control, rabbit polyclonal anti-IFNβ antibody, or normal rabbit serum for 1 hour at 37°C. The treated supernatants were then transferred to wells of confluent HFF fibroblasts and cultured for 24 hours. The conditioned medium was removed and the fresh HFF were challenged with CMVPT30-gfp. Fluorescent cells were then counted on day 10 post-infection (PI). The data shown is representative of 3 independent experiments. *** indicates P ≤ 0.001 compared to control.