Figure 6From: The internal initiation of translation in bovine viral diarrhea virus RNA depends on the presence of an RNA pseudoknot upstream of the initiation codonCompensatory Mutations and expression levels. Top: In M7 the M6 mutations introduced in stem 1 are compensated, restoring stem 1 integrity and giving rise to a sequence resembling BVDV genotype 2. In M8 nucleotide exchanges were made to compensate for mutations introduced into stem 2 of mutant plasmids M1. Bottom: Relative SEAP and luciferase expression values normalized to specific mRNA levels in BHK cells stably transfected with wild-type and pseudoknot mutant plasmids depicted in figures 5 and 6.Back to article page