Figure 3
From: Viroporin potential of the lentivirus lytic peptide (LLP) domains of the HIV-1 gp41 protein
LLP peptides partition into lipid bilayers. Fluorescence enhancement of tryptophan or NBD with partitioning of LLP peptides into lipid bilayers (a) LLP-1 (NBD), (b) LLP-2 (NBD), (c) LLP-1D (NBD), (d) 10%POPG:90%POPC (NBD) lipid alone controls (e) PO4 blanks (f) LLP-3, (g) 10%POPG:90%POPC (tryptophan) lipid alone controls. Partitioning of LLP peptides partitioning into lipid bilayers are presented as fluorescence enhancements in (h) and the results of curve fitting are shown in (i). In (j), the largest blue shift of the emission maxima for each peptide indicating transitions from aqueous to lipid environments are shown. A, B, C, and F represent lipid blank and PO4 blank subtracted spectra. H, I, and J are all calculated from these normalized spectra.