Figure 7

Isolation of insert from replicon RNA. A. RT-PCR product from C6/36–78 cells or naive C6/36 cells using primers BG162 and BG192. A non-specific amplification product was observed in C6/36 cells but not in C6/36–78 cells. B. Results of RT-PCR product recombination reactions transformed into E. coli. RT-PCR isolated cDNA was recombined into the pDONR222 entry plasmid and transformed into XL1-blue bacteria. C. Colony PCR from random isolated colonies from Figure 7B. The M13(-20)/M13 Rev primer set indicates the total insert size, and the M13(-20)/BG209 primer set detects the orientation of the GFP gene insert in the isolated plasmid.