Figure 6
From: Recombination-ready Sindbis replicon expression vectors for transgene expression
SINV replicons can express epitope tagged mosquito genes for several days. A. C6/36, S2, or BHK cells were infected with PIPs expressing a V5 epitope-tagged Ae. aegypti R2D2 cDNA either in a sense (pBG156) or antisense (pBG155) orientation. Cells were treated with blasticidin for four days and V5-R2D2 detected by western blot analysis. B. C6/36 cells were co-cultured with pBG156/pBG44 co-transfected BHK cells, transferred to new plates, and samples of cells collected at the indicated times for western blot analysis.