Figure 5From: Thiol-reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L1Sedimentation and morphology of the 16PVs that have bound to DTNB or NEM. (A) The 16PVs were incubated with DTNB (2 mM) or NEM (2 mM) at 37°C for 2 h. The sample was loaded on the top of a linear sucrose-density gradient (5 to 40%) and centrifuged. L1 in the fractions obtained by a bottom puncture was detected by immunoblotting with mouse anti-HPV16L1 antibody. (B) The 16PVs were incubated with NEM (2 mM) at 37°C for 2 h and observed under a transmission electron microscope.Back to article page