Figure 3From: Thiol-reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L1Binding of BPEOIA to L1 of the 16PV. (A) The 16PVs were incubated with 1 mM biotin-PEO-iodoacetamide (1 mM) in DMEM at 37°C for 2 h, electrophoresed on an SDS-polyacrylamide gel, and stained with SYPRO Ruby. (B) The proteins in the gel were transferred to a polyvinylidene difluoride membrane and probed with Streptavidin-HRP.Back to article page