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Figure 3 | Virology Journal

Figure 3

From: Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein

Figure 3

Role of the coiled coil region for interaction of NP with VP35. (A) Mutants of NP (Fig. 1) were in vitro translated and metabolically labeled with [35S]ProMix. Samples were separated by SDS-PAGE and analyzed using a BioImager. (B) In vitro translated and [35S]ProMix metabolically labeled mutants of NP (Fig. 1A) were incubated with bacterially expressed GST-VP35 or GST (negative control). Complexes were pulled down with glutathion-sepharose, separated on SDS-PAGE and analyzed using a BioImager. Binding of NP to GST-VP35 was set to 100%. (C) Quantification of 3 separate experiments as shown under (B).

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Virology Journal

ISSN: 1743-422X