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Figure 1 | Virology Journal

Figure 1

From: The role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type 1 in an inhibition assay

Figure 1

Sepharose CL-4B gel filtration of crude saliva. An aliquot (20 ml) of soluble human crude saliva extracted in 6 M GuHCl containing 10 mM EDTA, 5 mM NEM, 1 mM PMSF and 0.1% CHAPS pH 6.5 was chromatographed and eluted with 4 M GuHCl containing 10 mM EDTA, 5 mM NEM and 0.05% CHAPS pH 6.5 at flow rate of 48 ml/h at room temperature. Fractions were analysed for carbohydrate with PAS at 555 nm (ā—†) and for protein A280 (ā– ). Materials eluted in the void volume (Vo) and included volume (Vi) were pooled separately, dialysed against three changes of distilled water for overnight at 4Ā°C and freeze-dried.

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Virology Journal

ISSN: 1743-422X