Sequence analysis of sgJFH-1 replicons in mouse hepatocytes. (A) PCR primer sets used to amplify overlapping HCV DNA segments. (B) Twelve clones of each fragment were sequenced with an ABI automatic DNA sequencer. Mutations within viral proteins are indicated by the amino acid position of the mutation within the context of the full length HCV open reading frame. Mutations in the 3' UTR are designated by their nucleotide position in the context of the full length HCV genome.