Figure 5
From: Infection of human cytomegalovirus in cultured human gingival tissue
Growth of HCMV mutants ΔUS18, ΔRL9, and the parental TowneBAC in cultured cells (A) and gingival tissues (B). In (A), human foreskin fibroblasts (HFFs) (1 × 106 cells) were infected with each virus at a MOI of 0.05. At 0, 2, 4, 7, 10, and 14 days post infection, cells and culture media were harvested and sonicated. In (B), the tissues were infected with 2 × 104 PFU of each virus at the apical surface of the tissue. At 0, 3, 6, and 10 days post infection, the tissues were harvested, suspended in 10% milk, and sonicated. The viral titers were determined by plaque assays on HFFs. The limit of detection was 10 PFU/ml of the tissue homogenate. The values of the viral titer represent the average obtained from triplicate experiments. The standard deviation is indicated by the error bars.