Replication of HIV with PBS and A-loop complementary to tRNAGln. Panel A. Replication of wild type and viruses with PBS complementary to tRNAGln,1. Infections were established in SupT1 cells with equal amounts of virus as determined by infectious units. p24 antigen was then assayed in the culture supernatants at weekly intervals following initiation of the experiment. Values for the wild type virus increased to greater than 104 nanograms/ml by approximately 14 days following initiation of the infection. The cultures were terminated at day 28. Viruses derived from NL-4-Gln1 and NL-4-Gln1-AC were carried out to approximately 56 days post initiation of culture. Note that viruses derived from NL-4-Gln1-AC did not grow, as evidenced by p24 antigen that were near the levels of mock infected cells. Cultures were terminated at day 56. Data is representative from three independent experiments. Panel B. Replication of viruses with the PBS complementary to tRNAGln,3. The replication of the wild type virus is depicted. Cultures initiated with viruses derived from NL-4-Gln3 and NL-4-Gln3-AC were monitored over 56 days of culture. The viruses derived from NL-4-Gln3 eventually reached levels approximating that of the wild type virus by day 42 through 56. Viruses derived from NL-4-Gln3-AC demonstrated a slow and gradual increase reaching levels approximately 1/100 of that of the wild type virus at the time of termination of the culture (day 56). Data is representative of three independent experiments.