Figure 1

RT-PCR analysis of hBD-1, hBD-2 and hBD-3 mRNA expression in RRP papilloma samples from 15 different individuals ((Left Panel, Lanes 1–15) and normal oral mucosa tissue from 10 different individuals (Right Panel, Lanes 1–10). A housekeeping gene, β-actin (a), was detected as a 450 bp PCR product; hBD-1 (b), hBD-2 (c) and hBD-3 (d) expressions were detected as 108, 172 and 98 bp PCR products, respectively. Samples were separated by 2% agarose gel electrophoresis and stained with ethidium bromide. 100 bp-ladder molecular-weight markers are presented on the left of the panel. Negative controls including amplification without reverse transcriptase or with cDNA sample replaced by DNase, RNase – free, distilled water were performed in each experiment but are not shown due to limited gel space.