Figure 6

Influence of expression of cav-1 wild-type and dominant-negative mutant cav-1 on MLV-A titres in NIH3T3 cells. MLV-A infected NIH3T3 cells were transiently transfected with expression plasmids containing wt cav-1 cDNA or a dominant-negative mutant carrying to mutations in the scaffolding domain [28]. Titres were determined from supernatants 48 h after transfection on indicator cells according to Spearman and Karber as described in Materials and Methods. Normalized values are shown. In each of three independent experiments mock-transfected NIH-MLV-A were used for normalization. Standard deviations are shown.