SIV escape mutants in rhesus macaques vaccinated with NEF-derived lipopeptides and challenged with pathogenic SIVmac251

Table 1 Epitopic specificities found in 5 immunized macaques

Effector cells^a		% Specific lysis^c at the E/T ratio^d of
Macaque #	Target cells^b	100:1	33:1	11:1	4:1
92125	None	38	34	25	19
	NEF 169–178	86 ^e	87	70	54
92105	None	22	14	12	2
	NEF 128–136	34	24	13	5
92127	None		28	21
	NEF 116–126		43	32
92129	None	8	3	2	0
	NEF 128–136	46	34	28	9
	NEF 201–211	22	16	16	5
	NEF 211–219	19	16	10	4
	None	52	44	30
	NEF 169–178	65	54	33
92109	None	14	11	11	5
	NEF 101–110		26	22	16
	NEF 128–136	28	23
	None	41	34	41	27
	NEF 116–126	57	45	48	34
	None	25	21
	NEF 169–178	89	75
	None	14	11		5
	NEF 215–225	41	36		22
	None	19	9	7	3
	GAG -275	40	24	16	6

^a CTL cell lines were obtained from PBMCs of the 8 immunized macaques following specific-stimulation with the 7 long peptides in vitro.
^b Target cells were autologous B-LCLs immortalized by the herpes papio virus and incubated with short peptides (10μM).
^c Target cells (5×10³) were labeled with 51Cr and incubated for 4 h with various numbers of target cells.
^d E/T ratio, effector to target ratio.
^e CRT was considered positive if the specific-51Cr release observed against peptide-pulsed target cells exceeded that observed on B-LCLs without peptide by 10% at two E/T ratio.

ISSN: 1743-422X