Figure 4
From: Green fluorescent protein as a reporter of prion protein folding
Mutants M17, 22 and 25, selected by recovery of fluorescence, were grown and PrPc-GFP fusion protein present in the soluble (S) and insoluble (I) fractions of each induced culture after detergent lysis were resolved by 10% SDS-PAGE and probed with the prion monoclonal antibody 6H4. Reaction with mutant M22 has been largely lost due to degradation in the soluble phase and only residual insoluble material is detected.