Figure 2
From: Green fluorescent protein as a reporter of prion protein folding
Refolding of PrPc-GFP fusion proteins containing fragments from the prion amino terminal domain. A. 10% SDS-PAGE analysis of purified PrP-GFP fusion proteins encoding fragments from the N-terminus before (lanes 1–4) and after (5–8) refolding. Lanes 1 & 5, PrPc 23–156-GFP; lanes 2&6, PrPc 29–156-GFP; lanes 3&7, PrPc 23–169-GFP; lanes 4&8, PrPc 29–169-GFP. B. In vitro refolding kinetics of purified recombinant PrP-GFP fusion proteins; PrPc 23–156-GFP (◆); PrPc 29–156-GFP (●); PrPc 23–169-GFP(■) and PrPc 29–169-GFP(▲). Assays were done in duplicate and the average fluorescent units plotted against time. Fluorescence units are as recorded by the plate reader. The lower staining intensity of the refolded samples is due to dilution in the refolding buffer.