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Table 2 HSV-2 specific responses in the CD4 and CD8 cell populations. Number of ELISPOTs per 500,000 original PBMCs measured in samples depleted of either CD8 cells (designated CD4 cells) or depleted of CD4 cells (designated as CD8 cells) stimulated with various peptide pools in the IFN-γ ELISPOT assay. The peptide pools are described in Table 1 and the 1X dose of peptide is approximately 0.33 ug/ml of each individual peptide within the pool and the 10X dose is approximately 3.3 ug/ml of each peptide within the different pools. Control peptide is a single negative peptide. Sample was from subject 3.

From: Characterization of the IFN-γ T-cell responses to immediate early antigens in humans with genital herpes

 

CD4 cells

CD8 cells

Peptide pools

1X dose

10X dose

1X dose

10X dose

ICP27 all

15

2

0

0

ICP22 all

0

3

0

1

ICP0–1

0

2

0

0

ICP0–2

34

6

0

0

ICP0–3

6

0

0

1

ICP4–1/2

3

0

0

0

ICP4–3/4

45

26

0

43

ICP4–5/6

35

14

0

128

VP22

89

5

0

6

gD

47

25

0

0

Control peptide

0

0

0

0