Figure 4

Effect of peptide length on responses CD8 responses. CD8 cells isolated on magnetic beads from a subject with positive responses to VP22 were tested in the IFN-γ ELISPOT assay against peptides of various lengths each containing the putative RPRGEVRFL epitope. Results are averages of two experiments run in duplicate using CD8 cells harvested from 0.3 million PBMCs per well. Frozen PBMCs were used for this experiment.