Table 3 List of primers used to amplify the rep-containing HdIV segments by Polymerase Chain Reaction
HdIV segment | rep gene | forward primer (5'-3') | reverse primer (5'-3') |
|---|---|---|---|
SH-A2 | rep7 | ATCTTAAAGTGAGCACTATTGACGC | CCTGCGAAATTTCTTGATACACCACAGCCT |
SH-H | rep6 | AAGTGTTGCTTGACTCGGCT | TCAAGTCCAGGTTTCGGATC |
SH-J | rep5 | CTTGGTTACTCCAGCCCTTG | ACTCCTCCGAATAAAGGCGT |