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Table 1 Reproducibility of the SYBR® Green I dye-based qRT-PCR assay for the detection and quantification of Nipah virus RNA. Intra- and inter-assay variations were calculated using duplicates and at least 14 replicates, respectively.

From: Quantitative estimation of Nipah virus replication kinetics in vitro

CV (%) PFU/mL
  1 × 10-1 1 × 100 1 × 101 1 × 102 1 × 103 1 × 104 1 × 105 1 × 106
Intra-assay 3.27 1.20 1.67 0.06 0.43 0.01 0.79 0.96
Inter-assay 4.76 3.55 2.72 3.52 1.26 1.27 2.11 2.86