Schematic diagram of Lassa virus glycoprotein . The primary translation product preGP-C (aa 1–491), the signal peptide (SP) (aa 1–58), the precursor glycoprotein GP-C (aa 59–491) and the subunits GP-1 (aa 59–259) and GP-2 (260–491) are shown. Hydrophobic regions are indicated in stripes. The signal peptidase (SPase) cleavage site between threonine residues 58 and 59 (arrow), the SKI-1/S1P cleavage site C-terminally of leucine 259 (arrow) and the rabbit antiserum binding site, α-GP2 (aa 477–491) are indicated. Eleven potential N-glycosylation sites (tree-like symbols) were identified. Amino acid positions of potential N-glycosylation sites are shown.