Figure 3
From: Expression and processing of the Hepatitis E virus ORF1 nonstructural polyprotein
Effect of E-64d on ORF1 polyprotein processing. T. ni cells were infected with vORF1 for 12 hr at which time fresh medium containing 200 μM E-64d was added to the cells; an equal volume of DMSO served as the control. At 48 and 60 hr following E-64d addition, cells were harvested and the lysates analyzed by western blotting with either anti-His (A) or anti-FLAG (B) antibodies (lanes 1–4). Lysates from wild type AcMNPV infected cells were similarly analyzed at 48 hr after E-64d addition (lanes 5–6). In both parts, the upper and lower panels show results obtained following separation of the proteins on 7.5% and 12% SDS-polyacrylamide gels. The estimated fragment sizes are shown based on molecular size markers run on each gel (not shown).