Figure 1
From: Effect of Ebola virus proteins GP, NP and VP35 on VP40 VLP morphology
Protease Protection Assay of VLPs containing NP and VP40. VLP pellets were split into six fractions and treated with STE buffer (lane 1), soybean trypsin inhibitor (lane 2), Triton X-100 (lane 3), Trypsin (lane 4), Trypsin and Triton X-100 (lane 5), Trypsin and soybean trypsin inhibitor (lane 6). The fractions were divided equally, and viral proteins were immunoprecipitated with α-VP40 (Panel A), or α-NP (Panel B). Samples were resolved by SDS-PAGE and detected by autoradiography.