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Figure 1 | Virology Journal

Figure 1

From: Membrane-associated heparan sulfate is not required for rAAV-2 infection of human respiratory epithelia

Figure 1

Heparinase III treatment efficiently removes heparan sulfate from respiratory epithelial cell membranes. Immortalized epithelial cell lines fetal human tracheal (FHTE), human tracheal (HTE), and cystic fibrosis bronchial (IB3-1) were treated with increasing amounts of heparinase III for 60 minutes, incubated with Cy3-conjugated mouse anti-heparan sulfate antibody (10E4 epitope), and assessed for HSPG surface expression by flow cytometry. Each experiment was done in triplicate and expression was normalized to mean untreated expression level. Removal of surface HSPG plateaued after treatment with 10 mIU.

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Virology Journal

ISSN: 1743-422X